Jenson

Never wanted VAR and I absolutely fucking despise it.

If Robbie had gone down screaming and holding his foot, Atwell would have given a free kick to us. Bullshit decision.

This is Halton. Not sure the local data can be relied on too much. Up by about 1500 in a single day?

If this is a quote from the actual ruling “if someone is tested by PCR as positive when a threshold of 35 cycles or higher is used (as is the rule in most laboratories in Europe and the US), the probability that said person is infected is less than 3%, and the probability that said result is a false positive is 97%.” then the wording used is rather misleading based on the paper that the judgement is based on. From my reading of the study it seems that a positive PCR test using a Ct threshold of 35 cycles gives a positive accompanying culture result rate of 3%. That does not equal a false positive in terms of whether a person has the virus or not, as PCR is far more sensitive than any culture assay. It is certainly true that a PCR result is more robust with lower cycle numbers but used with appropriate controls any "false positive" should be easily identified. What the study says is that the higher the Ct value of a positive PCR test, the lower the probability that the person is contagious based on whether or not the virus can be cultured from the sample. This is a completely different parameter to what the PCR test does, which is to simply show that someone has live virus present at the time the swab was taken. This part of the article is bullshit: Even if the infectious viruses are long dead, a corona test can come back positive, because the PCR method multiplies even a tiny fraction of the viral genetic material enough The genetic material of the coronavirus is RNA, which would certainly not be present if the virus is "long dead". If the PCR gives a positive result it's because live virus was present.

I'm part of a few different groups, mates, footy lads, work etc and it's gone round all of them

It's all over WhatsApp who it's supposed to be.

https://www.liverpoolecho.co.uk/news/liverpool-news/joe-anderson-confirms-you-should-19264807 249 positive cases out of 42,233 asymptomatic people tested during the Liverpool testing trial over the weekend. So over 0.5% of those tested were walking around the city infected without knowing. Simple (if perhaps not scientific) extrapolation for the whole country meaning up to 335,000 current asymptomatic cases in the UK.

The trend on the Zoe app for the North West is looking a lot better.

The vaccine would be transported on dry ice which would keep it at a temperature of -80c

It's -80c, which is the required storage temperature for RNA. -80c freezers are commonplace so this really shouldn't be a problem.

We should have gone 433 when Bobby went off with Keita coming on instead of Shaq. It felt like we were being overrun in midfield the whole of that second half. Also, it might have been missed because De Bruyne stuck the pen wide but Allison dived the wrong way again. It's uncanny how he does it.

Does anyone seriously believe that the election was rigged in favour of the Democrats? Trump has been setting up this exact scenario for months now telling the world and his wife that mail-in ballots are corrupt and open to fraud.

Less than half an hour. Negative. Fuck you Coronavirus! It's not painful, the swab itself is quite thin. Going up the nose was ok for me, gagged a bit when I was swabbing the back of my throat. All in all though, nothing to it.

Just taken a test at the Uni as part of the Liverpool trial. Meant to be one the new lateral flow tests so I'll see how long it takes for the result to come back.

As much as I think Mane has probably outperformed him over the last year, there's nothing that gets me on the edge of my seat more than Mo running at the opposition defence.

Biden shortening in the odds a little

Ohio looking decent though

Miami is back in the game

Go Jacksonville

Some of the early numbers in Florida look good for Biden

Bit of a retro one

The PCR test will pick up live virus that is in your system. It won't tell you if you are infectious, and (this is a presumption) due to the nature of the swab tests it will not tell you how much live virus is there. (Note, I can and do use RT-PCR in a quantitative manner but I don't think it's possible in the way mass testing is being performed for covid).

As someone who uses RT-PCR regularly, it is an extremely accurate, sensitive and powerful technique when performed correctly, with well designed primers, proper controls and samples of sufficient quality. They can only be manipulated deliberately, will not detect other viruses if designed correctly and will not pick up dead virus as RNA as the genetic material is degraded extremely quickly. This is not to say that they are infallible but the shortcomings in the accuracy/sensitivity rates will be much more to do with how samples are taken and how the PCR is prepared, than any problem with the technique itself. I don't know the criteria that is applied to give a positive result but I can imagine that the quality of the actual swab tests taken is highly variable, and that when preparing the tests the need for speed will mean that the safeguards against contamination will be perhaps a little less stringent than I would use myself in the lab.

Love it.

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